Extraction and Purification of a Lectin from Red Kidney Bean and Preliminary Immune Function Studies of the Lectin and Four Chinese Herbal Polysaccharides
Figure 2
(a) Fractionation of the crude extract of red kidney bean lectin by DEAE Sephadex A-50 ion-exchange chromatography equilibrated with 0.1 M NaCl in 0.02 M Tris-HCl (pH 8.0). The column was washed initially with the same buffer to remove proteins that were not specifically absorbed to the column (data not shown on the figure) then washed with buffer in which the NaCl concentration increased linearly from 0.1 M to 1.0 M. Three peaks were obtained, among which peaks II and III exhibited hemagglutinating activity. Column: 2.6 cm; flow rate: 0.5 mL/min. (b) Fractionation of peak II on a Sephadex G-150 column with 0.15 M NaCl in 0.02 M Tris-HCl (pH 8.0). Column: 2.6cm; flow rate: 1 mL/min. Only the major peak (peak IV) exhibited hemagglutinating activity. Data represent (n = 6).
