BioMed Research International / 2010 / Article / Fig 1

Research Article

Trypsin Isoinhibitors with Antiproliferative Activity toward Leukemia Cells from Phaseolus vulgaris cv “White Cloud Bean”

Figure 1

Results of ion exchange chromatography on SP-Sepharose column ( 2 . 5 × 2 0  cm). The sample is fraction of white cloud bean extract previously adsorbed on DEAE-cellulose and eluted with 0.2 M NaCl added to the buffer, and subsequently unadsorbed on Affi-gel blue gel in the Tris-HCl buffer. Starting buffer for SP-Sepharose chromatography is 10 mM NH4OAc buffer (pH 5). Broken line across the right half of the chromatography represents the linear 0-1 M NaCl gradient in 10 mM N H 4 OAc buffer (pH 5) employed to elute adsorbed proteins. Trypsin inhibitory activity was found in both fractions SP2 and SP3.

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