Assessment of DNA Damage by RAPD in <i>Paracentrotus lividus</i> Embryos Exposed to Amniotic Fluid from Residents Living Close to Waste Landfill Sites

<table>Representative RAPD profiles showing DNA fingerprint patterns with DNA from 3 of 15 polluted amniotic liquid-treated (<svg height="10.325" id="M36" style="vertical-align:-0.0pt" version="1.1" viewbox="0 0 11.225 10.325" width="11.225" xmlns="http://www.w3.org/2000/svg">
<g transform="matrix(1.25,0,0,-1.25,0,10.325)">
<g transform="translate(72,-63.74)">
<text transform="matrix(1,0,0,-1,-71.95,63.79)">
<tspan style="font-size: 12.50px; " x="0" y="0">𝑇</tspan>
</text>
</g>
</g>
</svg>) and 1 of the 15 non-polluted amniotic liquid-treated sea urchin embryos (<svg height="10.9125" id="M37" style="vertical-align:-0.17554pt" version="1.1" viewbox="0 0 10.55 10.9125" width="10.55" xmlns="http://www.w3.org/2000/svg">
<g transform="matrix(1.25,0,0,-1.25,0,10.9125)">
<g transform="translate(72,-63.27)">
<text transform="matrix(1,0,0,-1,-71.95,63.5)">
<tspan style="font-size: 12.50px; " x="0" y="0">C</tspan>
</text>
</g>
</g>
</svg>).  Arrows indicate gains/losses differences for amplification products and the size of the amplified fragments (bp). Primers used (OPU8 and OPU14) are indicated at the bottom of each set of fingerprints. <svg height="10.475" id="M38" style="vertical-align:-0.0pt" version="1.1" viewbox="0 0 14.0125 10.475" width="14.0125" xmlns="http://www.w3.org/2000/svg">
<g transform="matrix(1.25,0,0,-1.25,0,10.475)">
<g transform="translate(72,-63.62)">
<text transform="matrix(1,0,0,-1,-71.95,63.67)">
<tspan style="font-size: 12.50px; " x="0" y="0">M</tspan>
</text>
</g>
</g>
</svg>, DNA molecular size marker, 100 bp  ladder.</table>

BioMed Research International

fig3

Figure 3

Figure 3: Assessment of DNA Damage by RAPD in <i>Paracentrotus lividus</i> Embryos Exposed to Amniotic Fluid from Residents Living Close to Waste Landfill Sites 

Figure 3 | Assessment of DNA Damage by RAPD in Paracentrotus lividus Embryos Exposed to Amniotic Fluid from Residents Living Close to Waste Landfill Sites 