The Expression of Soluble and Active Recombinant Haemophilus influenzae IgA1 Protease in E. coli

Table 1

IgA1 protease expression constructs.

Construct	Vector	Primer	Tag	Expression location
pET-S-IGAN	pET21a	IgA-NdeI-SS-5^':	C-terminal His tag	E. coli periplasm
		gctcatatgctaaataaaaaattcaaactc
		IgA-6his-BamHI-3^':
		caaggatcctagtggtggtggtggtggt
		gaggcacatcagcttgaatattattag
pET-IGAN	pET21a	IgA-NdeI-5^':	C-terminal His tag	E. coli cytoplasm
		gctcatatggcgttagtgagagacgatgtg
		IgA-6his-BamHI-3^':
		caaggatcctagtggtggtggtggtggt
		gaggcacatcagcttgaatattattag
pCold-S-IGAN	pCold IV	IgA-NdeI-SS-5^':	C-terminal His tag	E. coli periplasm
		gctcatatgctaaataaaaaattcaaactc
		IgA-6his-BamHI-3^':
		caaggatcctagtggtggtggtggtggt
		gaggcacatcagcttgaatattattag
pCold-IGAN	pCold IV	IgA-NdeI-5^':	C-terminal His tag	E. coli cytoplasm
		gctcatatggcgttagtgagagacgatgtg
		IgA-6his-BamHI-3^':
		caaggatcctagtggtggtggtggtggt
		gaggcacatcagcttgaatattattag