BioMed Research International / 2010 / Article / Tab 1 / Research Article
Aquaporin-6 Expression in the Cochlear Sensory Epithelium Is Downregulated by Salicylates Table 1 Primer sequences used for semiquantitative and real time reverse transcription/polymerase chain reaction (RT-PCR).
Gene Primer sequences Size (bp) References Accession number
𝛽
-actin*Forward
5
-CAGATCATGTTTGAGACCTT-
3
509 [18 ] NM_007393 Reverse
5
-CGGATGTCMACGTCACACTT-
3
AQP1 Forward
5
-TGCGTTCTGGCCACCACTGAC-
3
327 [19 ] NM_007472 Reverse
5
-GATGTCGTCAGCATCCAGGTC-
3
AQP2 Forward
5
-GTTGCCATGTCTCCTTCCTT-
3
441 This study NM_009699 Reverse
5
-GGAACAGCAGGTAGTTGTAG-
3
AQP4 Forward
5
-TTTCAAAGGAGTCTGGACTC-
3
383 [20 ] NM_009700 Reverse
5
-TTCCATGAACCGTGGTGACT-
3
AQP6 Forward
5
-CTGCCATGATTGGAACCTCT-
3
309 This study NM_175087 Reverse
5
-CTGTGTCCTCAGAGTTTGTC-
3
DQ_826418 AQP6a-b Forward
5
-ACTGGCTGTTCCATGAACCC-
3
727 (a), [14 ] NM_175087 Reverse
5
-AGGAAGTGGCCAGGAGGTACT-
3
364 (b) DQ_826418 AQP7 Forward
5
-TTTGCAGGCGGAGACCTGTT-
3
437 [21 ] NM_007473 Reverse
5
-CTCTGAGGATCCTGTGGTAT-
3
*In real time RT-PCR experiments
𝛽
-actin primers were Mm_Actb_2_SG, QuantiTect Primer Assay QT01136772, Qiagen. AQP6a-b, aquaporin-6 variants a and b. (a) and (b): AQP6 variant a and variant b.
