Autocrine, Not Paracrine, Interferon-Gamma Gene Delivery Enhances Ex Vivo Antigen-Specific Cytotoxic T Lymphocyte Stimulation and Killing
Chromosomal integration of AAV/IFN-γ vector DNA into DC and T cells. Cells were infected as described in the Materials and Methods section and then analyzed for proviral integration by, first, PCR amplification of the vector-chromosomal junctions, followed by agarose gel electrophoresis of the PCR products, Southern blotting and probing with labeled vector DNA. Each band represents a separate AAV integrant within the cell population. (a) shows the results from DC. (b) shows the results from T cells.
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