Figure 2: Viral Evolution in an Animal with Known Protective Mamu Alleles. (a) Comparison of IFN-γ ELISPOT values for the vaccinated, Mamu 𝐴 01 + NP animal 16040 before or after the Mamu 𝐴 01-restricted Gag CTPYDINQM to CIPYDINQM, Tat STPESANL to PTPESANP, and Pol STPPLVRLV to SSPPLVRLV mutations. Statistical analysis was performed using the Mann Whitney test. (b) NASBA viral loads and IFN- 𝛾 ELISPOT results for all three epitopes were plotted to determine the effects of mutations on the control of viral replication.