A Multifactorial Mechanism in the Superior Antimalarial Activity of -C-GalCer
Figure 4
-C-GalCer induces a prolonged in vivo maturation of DCs compared to -GalCer. Groups of 3 WT mice were injected i.p. with 1 g -GalCer or -C-GalCer, or with nothing, and 2, 6, or 24 hours later splenocytes were collected and subjected to FACS analysis. CD11c+ cells were gated and analyzed for their levels of (a) MHC class II, (b) CD86, and (c) CD40 at the different time points after glycolipid administration. Also shown are isotype control stainings obtained from the maximally activated, 24-hour, -GalCer-treated splenocyte populations, gated on CD11c+ cells. The numbers next to each histogram tracing represent the mean fluorescence intensity for that tracing. The data shown come from one of four experiments with similar results.
