Improved Method for In Vitro Secondary Amastigogenesis of Trypanosoma cruzi: Morphometrical and Molecular Analysis of Intermediate Developmental Forms
Figure 1
Transformation kinetics from tissue-derived trypomastigote to amastigote in hgDMEM at pH 5 without FBS at 37°C: relative percentage of trypomastigotes (-♦-), IFs (-■-), and amastigotes (-▲-) analyzed in fresh preparations under the light microscope. The results are the average of 3 independent experiments.