Research Article

Improved Method for In Vitro Secondary Amastigogenesis of Trypanosoma cruzi: Morphometrical and Molecular Analysis of Intermediate Developmental Forms

Figure 1

Transformation kinetics from tissue-derived trypomastigote to amastigote in hgDMEM at pH 5 without FBS at 37°C: relative percentage of trypomastigotes (--), IFs (-■-), and amastigotes (--) analyzed in fresh preparations under the light microscope. The results are the average of 3 independent experiments.
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