Neutrophils Compromise Retinal Pigment Epithelial Barrier Integrity

Figure 1

Neutrophil exposure increased explant permeability. (a) The kinetics of RPE explant permeability following neutrophil treatment. The basolateral side of the explant was incubated with neutrophils for 1 to 3 hours at 37^∘C. (b) Neutrophil-induced increase of permeability of the RPE explant. The basolateral side of the explant was incubated with neutrophils for 3 hours at 37^∘C. The apparent permeability was determined by [³H]-mannitol flux. The increased permeability induced by neutrophils is significant compared with control. *represents 𝑃<.05. Addition of PBS to the explant did not change the permeability.

(a)

(b)