Green Tea Epigallocatechin Gallate Exhibits Anticancer Effect in Human Pancreatic Carcinoma Cells via the Inhibition of Both Focal Adhesion Kinase and Insulin-Like Growth Factor-I Receptor
Figure 2
EGCG inhibits FAK activity. (a) Cells were plated at a density of 2 × 105 cells/ml in the presence of various concentrations of EGCG for 12 hours, except for BxPC-3 at 100 μM for additional 4 or 8 hours. Total cell lysates were subjected to western blot analysis with indicated antibodies. Cells cultured in medium with 0.1% DMSO were used as a control. (b) Immunofluorescence staining for p-FAK (green) in BxPC-3 cells in the absence or presence of 100 μM EGCG, as indicated. Expression of p-FAK (arrows) was significantly reduced in EGCG-treated cells as compared with control cells. Staining with TOPRO-3 (red) visualizes the nuclei.
