Green Tea Epigallocatechin Gallate Exhibits Anticancer Effect in Human Pancreatic Carcinoma Cells via the Inhibition of Both Focal Adhesion Kinase and Insulin-Like Growth Factor-I Receptor
Figure 4
(A) EGCG fails to inhibit phosphorylation of MAPK and mTOR in AsPC-1 and BxPC-3 cells. Cells were plated at a density of 2 × 105 cells/ml in the presence of various concentrations of EGCG for 12 hours, except for BxPC-3 at 100 μM for additional 4 or 8 hours. Total cell lysates were subjected to western blot analysis. (b) MAPK inhibitor enhances EGCG-induced antiproliferative response in BxPC-3 cells. Cells were plated at a density of 0.6 × 105 cells/ml in 24-well dishes with different concentrations of EGCG for 48 hours in the presence or absence of 10 μM U0126 (MEK1/2 inhibitor). Cells were detached by trypsin, stained with trypan blue and counted manually using a hemacytometer. Values were obtained from experiments conducted in triplicate. Asterisk indicates significant difference. Inhibition of phosphorylation of MAPK by U0126 at 10 μM was confirmed with western blot analysis.
