Biochemical Signatures of Doppel Protein in Human Astrocytomas to Support Prediction in Tumor Malignancy
Figure 1
Identification and cellular localization of Dpl in testis and astrocytoma samples. Immunoblotting analysis of cytoplasmic (a) and microsomal (b) fractions. To limit the figure size, only positive samples have been reported. Dpl was detected with the anti-Dpl rabbit antiserum (Dpl-Val) previously described in[10, 11], loading 20 g of purified proteins. Each astrocytoma sample is identified by a progressive number followed by their WHO grade of malignancy (1/I, 2/II, etc.). (c) Distribution of Dpl in low- and high-grade astrocytoma samples as a cytoplasmic fraction (black bars), microsomal fraction (white bars) and in both fractions (grey bars). (d) Distribution of microsomal Dpl in low- and high-grade astrocytoma samples, regardless of the presence/absence of the cytosolic protein. Asterisks indicated statistically significant Anova One-way probabilities ().
