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Journal of Biomedicine and Biotechnology
Volume 2010, Article ID 301067, 9 pages
http://dx.doi.org/10.1155/2010/301067
Research Article

Biochemical Signatures of Doppel Protein in Human Astrocytomas to Support Prediction in Tumor Malignancy

1Dipartimento di Biochimica, “A. Castellani”, University of Pavia, Via Taramelli 3/b, 27100 Pavia, Italy
2Dipartimento di Genetica e Microbiologia, “A. Buzzati-Traverso”, University of Pavia, Via Ferrata 1, 27100 Pavia, Italy
3IRCCS Policlinico S. Matteo, Via Ferrata 1, 27100 Pavia, Italy
4IGM-CNR, Via Ferrata 1, 27100 Pavia, Italy
5Dipartimento di Patologia Umana e Oncologia, University of Siena, via Banchi di Sotto 55, 53100 Siena, Italy

Received 10 June 2010; Revised 9 September 2010; Accepted 17 September 2010

Academic Editor: Paul Harrison

Copyright © 2010 Paola Rognoni et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Linked References

  1. P. Kleihues and W. K. Cavenee, Eds., World Health Organization Classification of Tumours of The Nervous System, IARC Press, Lyon, France, 2000.
  2. H. Ohgaki and P. Kleihues, “Genetic pathways to primary and secondary glioblastoma,” American Journal of Pathology, vol. 170, no. 5, pp. 1445–1453, 2007. View at Publisher · View at Google Scholar · View at Scopus
  3. C. Dai and E. C. Holland, “Glioma models,” Biochimica et Biophysica Acta, vol. 1551, no. 1, pp. M19–M27, 2001. View at Publisher · View at Google Scholar · View at Scopus
  4. T. F. Ørntoft and E. M. Vestergaard, “Clinical aspects of altered glycosylation of glycoproteins in cancer,” Electrophoresis, vol. 20, no. 2, pp. 362–371, 1999. View at Google Scholar · View at Scopus
  5. R. Chen, J. R. Eshleman, R. A. Brodsky, and M. E. Medof, “Glycophosphatidylinositol-anchored protein deficiency as a marker of Mutator phenotypes in cancer,” Cancer Research, vol. 61, no. 2, pp. 654–658, 2001. View at Google Scholar · View at Scopus
  6. T. W. Vogel, Z. Zhuang, J. Li et al., “Proteins and protein pattern differences between glioma cell lines and glioblastoma multiforme,” Clinical Cancer Research, vol. 11, no. 10, pp. 3624–3632, 2005. View at Publisher · View at Google Scholar · View at Scopus
  7. R. C. Moore, I. Y. Lee, G. L. Silverman et al., “Ataxia in prion protein (PrP)-deficient mice is associated with upregulation of the novel PrP-like protein doppel,” Journal of Molecular Biology, vol. 292, no. 4, pp. 797–817, 1999. View at Publisher · View at Google Scholar · View at Scopus
  8. S. Comincini, V. Ferrara, A. Arias et al., “Diagnostic value of PRND gene expression profiles in astrocytomas: relationship to tumor grades of malignancy,” Oncology Reports, vol. 17, no. 5, pp. 989–996, 2007. View at Google Scholar · View at Scopus
  9. S. Comincini, A. Facoetti, I. Del Vecchio et al., “Differential expression of the prion-like protein doppel gene (PRND) in astrocytomas: a new molecular marker potentially involved in tumor progression,” Anticancer Research, vol. 24, no. 3A, pp. 1507–1517, 2004. View at Google Scholar · View at Scopus
  10. S. Comincini, L. R. Chiarelli, P. Zelini et al., “Nuclear mRNA retention and aberrant doppel protein expression in human astrocytic tumor cells,” Oncology Reports, vol. 16, no. 6, pp. 1325–1332, 2006. View at Google Scholar · View at Scopus
  11. E. Sbalchiero, A. Azzalin, S. Palumbo et al., “Altered cellular distribution and sub-cellular sorting of doppel (Dpl) protein in human astrocytoma cell lines,” Cellular Oncology, vol. 30, no. 4, pp. 337–347, 2008. View at Publisher · View at Google Scholar · View at Scopus
  12. G. L. Silverman, K. Qin, R. C. Moore et al., “Doppel is an N-glycosylated, glycosylphosphatidylinositol-anchored protein: expression in testis and ectopic production in the brains of Prnp(0/0) mice predisposed to purkinje cell loss,” Journal of Biological Chemistry, vol. 275, no. 35, pp. 26834–26841, 2000. View at Publisher · View at Google Scholar · View at Scopus
  13. A. Behrens, “Physiological and pathological functions of the prion protein homologue Dpl,” British Medical Bulletin, vol. 66, pp. 35–42, 2003. View at Publisher · View at Google Scholar · View at Scopus
  14. K. Peoc'h, C. Serres, Y. Frobert et al., “The human “prion-like” protein Doppel is expressed in both sertoli cells and spermatozoa,” Journal of Biological Chemistry, vol. 277, no. 45, pp. 43071–43078, 2002. View at Publisher · View at Google Scholar · View at Scopus
  15. A. Behrens, N. Genoud, H. Naumann et al., “Absence of the prion protein homologue Doppel causes male sterility,” EMBO Journal, vol. 21, no. 14, pp. 3652–3658, 2002. View at Publisher · View at Google Scholar · View at Scopus
  16. A. Kornberg, “Lactic dehydrogenase of muscle : Pyruvate + DPNH Lactate + DPN,” in Methods in Enzymology, vol. 1, pp. 441–443, Academic Press, New York, NY, USA, 1955. View at Google Scholar
  17. R. C. Nordlie and W. J. Arion, “Glucose-6-phosphatase,” in Methods in Enzymology, vol. 9, pp. 619–625, Academic Press, New York, NY, USA, 1966. View at Google Scholar
  18. O. H. Lowry, N. J. Rosebrough, A. L. Farr, and R. J. Randall, “Protein measurement with the Folin phenol reagent,” The Journal of Biological Chemistry, vol. 193, no. 1, pp. 265–275, 1951. View at Google Scholar
  19. A. S. B. Edge, C. R. Faltynek, L. Hof, L. E. Reichert Jr., and P. Weber, “Deglycosylation of glycoproteins by trifluoromethanesulfonic acid,” Analytical Biochemistry, vol. 118, no. 1, pp. 131–137, 1981. View at Google Scholar · View at Scopus
  20. A. Görg, W. Weiss, and M. J. Dunn, “Current two-dimensional electrophoresis technology for proteomics,” Proteomics, vol. 4, no. 12, pp. 3665–3685, 2004. View at Publisher · View at Google Scholar · View at Scopus
  21. H. Mo, R. C. Moore, F. E. Cohen et al., “Two different neurodegenerative diseases caused by proteins with similar structures,” Proceedings of the National Academy of Sciences of the United States of America, vol. 98, no. 5, pp. 2352–2357, 2001. View at Publisher · View at Google Scholar · View at Scopus
  22. J. W. Dennis, M. Granovsky, and C. E. Warren, “Glycoprotein glycosylation and cancer progression,” Biochimica et Biophysica Acta, vol. 1473, no. 1, pp. 21–34, 1999. View at Publisher · View at Google Scholar · View at Scopus