Electrodelivery of Drugs into Cancer Cells in the Presence of Poloxamer 188

Figure 1

MTT test of treated MDA-MB-231 cells at 24 hoursours after treatment; 5⋅10⁵ cells suspended in 100 𝜇l of a buffer (0.3 M mannitol, 0.1 mM Ca acetate and 0.1 mM Mg acetate) were electroporated according to the following electrical parameters: field strength 1.0 kV⋅cm⁻¹ and one rectangular pulse with a duration of 5 ms. Cisplatin was used at 10 𝜇M and bleomycin—at 20 𝜇M concentration. +p indicates electroporated probes, +P188 indicates that cells were electroporated in the presence of 0.1 mM P188, and +|P188 (0.1 mM) indicates the addition of P188 after the electrical pulse. All probes were diluted under identical conditions. The data represent the mean values from three experiments.