317545.fig.004a
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317545.fig.004b
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Figure 4: Complete VLP internalize in vivo in the ileum (a) and colon (b). Frozen sections of intestinal samples from mice administered with complete GFP-VLP (green) were labeled with anti-RF antibodies and Cy3-conjugated (red); FITC-lectin DSA was used as a positive control. Tissue sections were analyzed by confocal microscopy. Overlay analysis shows that GFP colocalizes with viral proteins (yellow/orange). The arrows indicate the VLP. Representative images of 3 experiments are shown. Scale bar 20  𝜇 m.