Improved Killing of Human High-Grade Glioma Cells by Combining Ionizing Radiation with Oncolytic Parvovirus H-1 Infection
Figure 1
Effects of late parvovirus H-1 (H-1PV) infection on human high-grade glioma cells surviving ionizing radiation (IR). Short-term cultures of human gliosarcoma NCH-37, human glioblastoma NCH-82, and human glioblastoma NCH-89 were seeded at 30,000 cells/well, irradiated with 10 Gy and infected with H-1PV at an MOI of 5 PFU/cell 9 days post-IR (MOI 5) and compared to mock-infected cells surviving IR (MOCK). The short-term culture of in vivo irradiated recurrent glioblastoma NCH-307 was seeded at 30,000 cells/well and infected with H-1PV at low ( PFU/cell: MOI 5) or high ( PFU/cell: MOI 100) virus doses and compared to mock-infected cells. All experiments were performed in three independent assays. Viability (%) was assessed as the number of living treated cells over the number of living untreated cells three days post (mock-) infection. Error bars represent the respective standard error. (*) indicates significant differences () between the number of living infected and the corresponding number of living MOCK-infected cells.
