Molecular Characterization of Heterologous HIV-1gp120 Gene Expression Disruption in <i>Mycobacterium bovis</i> BCG Host Strain: A Critical Issue for Engineering Mycobacterial Based-Vaccine Vectors

<table>PCR analysis of the HIV-1gp120 gene cloned into pMV261, pJH222 and pJH223 vectors. <i>Lane 1, 3 and 5 </i>are PCR products of the HIV-1gp120 gene (1578 bp) cloned into pMV261 (<i>lane 1</i>), pJH222 (<i>lane 3</i>) and pJH223 (<i>lane 5</i>) vectors, respectively. Plasmid DNA before BCG transformation (pre-BCG) were used as positive controls (lane 1, 3 and 5). <i>Lane 2, 4 and 6 </i>are PCR products from the rBCG:HIV-1gp120 (pMV261), rBCG:HIV-1gp120 (pJH222) and rBCG:HIV-1gp120 (pJH223) colonies.</table>

BioMed Research International

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Figure 1

Figure 1: Molecular Characterization of Heterologous HIV-1gp120 Gene Expression Disruption in <i>Mycobacterium bovis</i> BCG Host Strain: A Critical Issue for Engineering Mycobacterial Based-Vaccine Vectors 

Figure 1 | Molecular Characterization of Heterologous HIV-1gp120 Gene Expression Disruption in Mycobacterium bovis BCG Host Strain: A Critical Issue for Engineering Mycobacterial Based-Vaccine Vectors 