Molecular Characterization of Heterologous HIV-1gp120 Gene Expression Disruption in <i>Mycobacterium bovis</i> BCG Host Strain: A Critical Issue for Engineering Mycobacterial Based-Vaccine Vectors

<table>Multiple HIV-1gp120 DNA sequence alignment carrying the <svg height="5.7874999" id="M59" style="vertical-align:-0.0pt" version="1.1" viewbox="0 0 10.825 5.7874999" width="10.825" xmlns="http://www.w3.org/2000/svg">
<g transform="matrix(1.25,0,0,-1.25,0,5.7875)">
<g transform="translate(72,-67.37)">
<text transform="matrix(1,0,0,-1,-71.95,67.42)">
<tspan style="font-size: 12.50px; " x="0" y="0">∼</tspan>
</text>
</g>
</g>
</svg>900-bp deletion from the mutant rBCG:HIV-1gp120 (pMV261) colonies. HIV-1gp120 DNA sequences of 10 mutant rBCG:HIV-1gp120 (pMV261) colonies were aligned and compared with the parental nucleotide sequence of the HIV-1gp120 gene. The <svg height="5.7874999" id="M60" style="vertical-align:-0.0pt" version="1.1" viewbox="0 0 10.825 5.7874999" width="10.825" xmlns="http://www.w3.org/2000/svg">
<g transform="matrix(1.25,0,0,-1.25,0,5.7875)">
<g transform="translate(72,-67.37)">
<text transform="matrix(1,0,0,-1,-71.95,67.42)">
<tspan style="font-size: 12.50px; " x="0" y="0">∼</tspan>
</text>
</g>
</g>
</svg>900 bp deleted fragment (sequence and position shown in the scheme) structurally corresponds to the gp120 core (see protein translation).</table>

BioMed Research International

fig3

Figure 3

Figure 3: Molecular Characterization of Heterologous HIV-1gp120 Gene Expression Disruption in <i>Mycobacterium bovis</i> BCG Host Strain: A Critical Issue for Engineering Mycobacterial Based-Vaccine Vectors 

Figure 3 | Molecular Characterization of Heterologous HIV-1gp120 Gene Expression Disruption in Mycobacterium bovis BCG Host Strain: A Critical Issue for Engineering Mycobacterial Based-Vaccine Vectors 