Molecular Characterization of Heterologous HIV-1gp120 Gene Expression Disruption in Mycobacterium bovis BCG Host Strain: A Critical Issue for Engineering Mycobacterial Based-Vaccine Vectors

Table 3

Constructs obtained using E. coli/mycobacterial shuttle expression vectors to express HIV-1gp120 antigen (HXBc2).

Constructs	Promoter	Secretion signal	Resistance Gene	Complementing gene	Tag
pMV261:HIV-1 gp120	BCG hsp60	None	Kanamycin	None	HA
					His
pJH222:HIV-1 gp120	𝛼-antigen of Mycobacterium spp	19 kDa lipoprotein (M. tuberculosis)	Kanamycin	Lysine	HA
					His
pJH223:HIV-1 gp120	𝛼-antigen of Mycobacterium spp	19 kDa lipoprotein (M. tuberculosis)	Kanamycin	Lysine	HA
					His

HA: hemagglutinin epitope tag from influenza virus; His: Histidine residues.