The Collagen V Homotrimer <svg     style="vertical-align:-4.49648pt;width:78.824997px;" id="M1" height="21.637501" version="1.1" viewBox="0 0 78.824997 21.637501" width="78.824997"  xmlns:xlink="http://www.w3.org/1999/xlink" xmlns="http://www.w3.org/2000/svg">
	
		
			<g transform="matrix(.022,-0,0,-.022,.062,15.975)"><path id="x5B" d="M290 -163h-170v866h170v-28q-79 -7 -94 -19.5t-15 -72.5v-627q0 -59 14.5 -71.5t94.5 -19.5v-28z" /></g><g transform="matrix(.022,-0,0,-.022,7.796,15.975)"><path id="x1D6FC" d="M545 106q-67 -118 -134 -118q-24 0 -40 37.5t-30 129.5h-2q-47 -72 -103 -119.5t-108 -47.5q-47 0 -76 45.5t-29 119.5q0 113 85 204t174 91q47 0 70 -33.5t43 -119.5h3q32 47 80 140l55 13l10 -9q-47 -80 -138 -201q17 -99 27.5 -136t22.5 -37q23 0 69 61zM333 204
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			<g transform="matrix(.016,-0,0,-.016,70.288,21.362)"><path id="x1D7D1" d="M58 524l-21 12q80 152 229 152q73 0 119 -41q45 -41 45 -96q0 -40 -21.5 -74.5t-62.5 -52.5q57 -20 89.5 -68.5t32.5 -109.5q0 -110 -86 -185t-214 -75q-69 0 -110.5 24.5t-41.5 65.5q0 26 18.5 43t46.5 17q45 0 96 -57q39 -43 79 -43q45 0 71.5 34t26.5 88q0 75 -49 121
q-25 23 -56 39q-34 17 -97 38v17q69 21 100 53t31 78q0 103 -99 103q-40 0 -69.5 -19t-56.5 -64z" /></g>

		
	
</svg> Production Is Unexpectedly Favored over the Heterotrimer <svg     style="vertical-align:-4.32007pt;width:133.58749px;" id="M2" height="21.424999" version="1.1" viewBox="0 0 133.58749 21.424999" width="133.58749"  xmlns:xlink="http://www.w3.org/1999/xlink" xmlns="http://www.w3.org/2000/svg">
	
		
			<g transform="matrix(.022,-0,0,-.022,.062,15.975)"><use xlink:href="#x5B"/></g><g transform="matrix(.022,-0,0,-.022,7.796,15.975)"><use xlink:href="#x1D6FC"/></g><g transform="matrix(.022,-0,0,-.022,20.529,15.975)"><use xlink:href="#x31"/></g><g transform="matrix(.022,-0,0,-.022,31.288,15.975)"><use xlink:href="#x28"/></g><g transform="matrix(.022,-0,0,-.022,39.044,15.975)"><use xlink:href="#x56"/></g><g transform="matrix(.022,-0,0,-.022,54.803,15.975)"><use xlink:href="#x29"/></g><g transform="matrix(.022,-0,0,-.022,62.559,15.975)"><use xlink:href="#x5D"/></g>

			<g transform="matrix(.016,-0,0,-.016,70.288,21.363)"><path id="x1D7D0" d="M453 211h25l-46 -211h-415v23q97 102 151 166q42 49 81 110q51 78 51 148q0 56 -31.5 91.5t-87.5 35.5q-77 0 -122 -90h-28q67 204 223 204q82 0 132 -49.5t50 -133.5q0 -110 -114 -218l-162 -154h140q82 0 107 13q27 13 46 65z" /></g>

			<g transform="matrix(.022,-0,0,-.022,78.762,15.975)"><use xlink:href="#x1D6FC"/></g><g transform="matrix(.022,-0,0,-.022,91.495,15.975)"><path id="x32" d="M412 140l28 -9q0 -2 -35 -131h-373v23q112 112 161 170q59 70 92 127t33 115q0 63 -31 98t-86 35q-75 0 -137 -93l-22 20l57 81q55 59 135 59q69 0 118.5 -46.5t49.5 -122.5q0 -62 -29.5 -114t-102.5 -130l-141 -149h186q42 0 58.5 10.5t38.5 56.5z" /></g><g transform="matrix(.022,-0,0,-.022,102.255,15.975)"><use xlink:href="#x28"/></g><g transform="matrix(.022,-0,0,-.022,110.011,15.975)"><use xlink:href="#x56"/></g><g transform="matrix(.022,-0,0,-.022,125.769,15.975)"><use xlink:href="#x29"/></g>

		
	
</svg> in Recombinant Expression Systems

<table class="table-group" id="tab1"><tr><td><table class="table"><tr><td class="thead-hr" colspan="3"><hr/></td></tr><tr class="thead"><td align="left">Constructs</td><td align="left">Host cells</td><td align="left">Recombinant yeast strains</td></tr><tr><td class="thead-hr" colspan="3"><hr/></td></tr><tr><td align="left">pPICZ<i>α</i>-C5A1N</td><td align="left"><i>P. pastoris</i> X-33</td><td align="left"><svg height="7.1750002" id="M122" style="vertical-align:-0.1254pt" version="1.1" viewbox="0 0 8.9375 7.1750002" width="8.9375" xmlns="http://www.w3.org/2000/svg">
<g transform="matrix(1.25,0,0,-1.25,0,7.175)">
<g transform="translate(72,-66.26)">
<text transform="matrix(1,0,0,-1,-71.95,66.44)">
<tspan style="font-size: 12.50px; " x="0" y="0">𝛼</tspan>
</text>
</g>
</g>
</svg>1(V) chain with <svg height="7.1750002" id="M123" style="vertical-align:-0.1254pt" version="1.1" viewbox="0 0 8.9375 7.1750002" width="8.9375" xmlns="http://www.w3.org/2000/svg">
<g transform="matrix(1.25,0,0,-1.25,0,7.175)">
<g transform="translate(72,-66.26)">
<text transform="matrix(1,0,0,-1,-71.95,66.44)">
<tspan style="font-size: 12.50px; " x="0" y="0">𝛼</tspan>
</text>
</g>
</g>
</svg>MF signal, without C-propeptide</td></tr><tr><td align="left">pPIC3.5K-C5A1M</td><td align="left"><i>P. pastoris</i> yJC300</td><td align="left">full length prepro-<svg height="7.1750002" id="M124" style="vertical-align:-0.1254pt" version="1.1" viewbox="0 0 8.9375 7.1750002" width="8.9375" xmlns="http://www.w3.org/2000/svg">
<g transform="matrix(1.25,0,0,-1.25,0,7.175)">
<g transform="translate(72,-66.26)">
<text transform="matrix(1,0,0,-1,-71.95,66.44)">
<tspan style="font-size: 12.50px; " x="0" y="0">𝛼</tspan>
</text>
</g>
</g>
</svg>1(V) chain</td></tr><tr><td align="left">pPICZ-C5A2</td><td align="left"><i>P. pastoris</i> yJC300</td><td align="left">full length prepro-<svg height="7.1750002" id="M125" style="vertical-align:-0.1254pt" version="1.1" viewbox="0 0 8.9375 7.1750002" width="8.9375" xmlns="http://www.w3.org/2000/svg">
<g transform="matrix(1.25,0,0,-1.25,0,7.175)">
<g transform="translate(72,-66.26)">
<text transform="matrix(1,0,0,-1,-71.95,66.44)">
<tspan style="font-size: 12.50px; " x="0" y="0">𝛼</tspan>
</text>
</g>
</g>
</svg>2(V) chain</td></tr><tr class="table-tr"><td colspan="3"><hr class="tbody-hr"/></td></tr></table></td></tr></table>

Yeast expression constructs, host cells, and recombinant strains.

BioMed Research International

tab1

Table 1

Table 1: The Collagen V Homotrimer <svg     style="vertical-align:-4.49648pt;width:78.824997px;" id="M1" height="21.637501" version="1.1" viewBox="0 0 78.824997 21.637501" width="78.824997"  xmlns:xlink="http://www.w3.org/1999/xlink" xmlns="http://www.w3.org/2000/svg">
	
		
			<g transform="matrix(.022,-0,0,-.022,.062,15.975)"><path id="x5B" d="M290 -163h-170v866h170v-28q-79 -7 -94 -19.5t-15 -72.5v-627q0 -59 14.5 -71.5t94.5 -19.5v-28z" /></g><g transform="matrix(.022,-0,0,-.022,7.796,15.975)"><path id="x1D6FC" d="M545 106q-67 -118 -134 -118q-24 0 -40 37.5t-30 129.5h-2q-47 -72 -103 -119.5t-108 -47.5q-47 0 -76 45.5t-29 119.5q0 113 85 204t174 91q47 0 70 -33.5t43 -119.5h3q32 47 80 140l55 13l10 -9q-47 -80 -138 -201q17 -99 27.5 -136t22.5 -37q23 0 69 61zM333 204
q-14 98 -31 149.5t-50 51.5q-49 0 -94 -70t-45 -164q0 -55 15.5 -86t40.5 -31q70 0 164 150z" /></g><g transform="matrix(.022,-0,0,-.022,20.529,15.975)"><path id="x31" d="M384 0h-275v27q67 5 81.5 18.5t14.5 68.5v385q0 38 -7.5 47.5t-40.5 10.5l-48 2v24q85 15 178 52v-521q0 -55 14.5 -68.5t82.5 -18.5v-27z" /></g><g transform="matrix(.022,-0,0,-.022,31.288,15.975)"><path id="x28" d="M300 -147l-18 -23q-106 71 -159 185.5t-53 254.5v1q0 139 53 252.5t159 186.5l18 -24q-74 -62 -115.5 -173.5t-41.5 -242.5q0 -130 41.5 -242.5t115.5 -174.5z" /></g><g transform="matrix(.022,-0,0,-.022,39.044,15.975)"><path id="x56" d="M687 650v-28q-52 -6 -71.5 -23t-47.5 -83q-142 -346 -208 -527h-31q-119 325 -207 545q-21 54 -39.5 68.5t-65.5 19.5v28h245v-28q-52 -5 -61.5 -15.5t3.5 -43.5q55 -157 166 -437h2q120 303 158 421q14 43 3.5 55.5t-71.5 19.5v28h225z" /></g><g transform="matrix(.022,-0,0,-.022,54.803,15.975)"><path id="x29" d="M275 270q0 -296 -211 -440l-19 23q75 62 116.5 174t41.5 243t-42 243t-116 173l19 24q211 -144 211 -440z" /></g><g transform="matrix(.022,-0,0,-.022,62.559,15.975)"><path id="x5D" d="M226 -163h-170v27q79 7 94 20t15 73v627q0 59 -15 72t-94 20v27h170v-866z" /></g>

			<g transform="matrix(.016,-0,0,-.016,70.288,21.362)"><path id="x1D7D1" d="M58 524l-21 12q80 152 229 152q73 0 119 -41q45 -41 45 -96q0 -40 -21.5 -74.5t-62.5 -52.5q57 -20 89.5 -68.5t32.5 -109.5q0 -110 -86 -185t-214 -75q-69 0 -110.5 24.5t-41.5 65.5q0 26 18.5 43t46.5 17q45 0 96 -57q39 -43 79 -43q45 0 71.5 34t26.5 88q0 75 -49 121
q-25 23 -56 39q-34 17 -97 38v17q69 21 100 53t31 78q0 103 -99 103q-40 0 -69.5 -19t-56.5 -64z" /></g>

		
	
</svg> Production Is Unexpectedly Favored over the Heterotrimer <svg     style="vertical-align:-4.32007pt;width:133.58749px;" id="M2" height="21.424999" version="1.1" viewBox="0 0 133.58749 21.424999" width="133.58749"  xmlns:xlink="http://www.w3.org/1999/xlink" xmlns="http://www.w3.org/2000/svg">
	
		
			<g transform="matrix(.022,-0,0,-.022,.062,15.975)"><use xlink:href="#x5B"/></g><g transform="matrix(.022,-0,0,-.022,7.796,15.975)"><use xlink:href="#x1D6FC"/></g><g transform="matrix(.022,-0,0,-.022,20.529,15.975)"><use xlink:href="#x31"/></g><g transform="matrix(.022,-0,0,-.022,31.288,15.975)"><use xlink:href="#x28"/></g><g transform="matrix(.022,-0,0,-.022,39.044,15.975)"><use xlink:href="#x56"/></g><g transform="matrix(.022,-0,0,-.022,54.803,15.975)"><use xlink:href="#x29"/></g><g transform="matrix(.022,-0,0,-.022,62.559,15.975)"><use xlink:href="#x5D"/></g>

			<g transform="matrix(.016,-0,0,-.016,70.288,21.363)"><path id="x1D7D0" d="M453 211h25l-46 -211h-415v23q97 102 151 166q42 49 81 110q51 78 51 148q0 56 -31.5 91.5t-87.5 35.5q-77 0 -122 -90h-28q67 204 223 204q82 0 132 -49.5t50 -133.5q0 -110 -114 -218l-162 -154h140q82 0 107 13q27 13 46 65z" /></g>

			<g transform="matrix(.022,-0,0,-.022,78.762,15.975)"><use xlink:href="#x1D6FC"/></g><g transform="matrix(.022,-0,0,-.022,91.495,15.975)"><path id="x32" d="M412 140l28 -9q0 -2 -35 -131h-373v23q112 112 161 170q59 70 92 127t33 115q0 63 -31 98t-86 35q-75 0 -137 -93l-22 20l57 81q55 59 135 59q69 0 118.5 -46.5t49.5 -122.5q0 -62 -29.5 -114t-102.5 -130l-141 -149h186q42 0 58.5 10.5t38.5 56.5z" /></g><g transform="matrix(.022,-0,0,-.022,102.255,15.975)"><use xlink:href="#x28"/></g><g transform="matrix(.022,-0,0,-.022,110.011,15.975)"><use xlink:href="#x56"/></g><g transform="matrix(.022,-0,0,-.022,125.769,15.975)"><use xlink:href="#x29"/></g>

		
	
</svg> in Recombinant Expression Systems 

Table 1 | The Collagen V Homotrimer 
	
		
			

			

		
	
 Production Is Unexpectedly Favored over the Heterotrimer 
	
		
			

			

			

		
	
 in Recombinant Expression Systems 

BioMed Research International

The Collagen V Homotrimer Production Is Unexpectedly Favored over the Heterotrimer in Recombinant Expression Systems

Table 1