The Collagen V Homotrimer Production Is Unexpectedly Favored over the Heterotrimer in Recombinant Expression Systems
Table 1
Yeast expression constructs, host cells, and recombinant strains.
Constructs
Host cells
Recombinant yeast strains
pPICZα-C5A1N
P. pastoris X-33
1(V) chain with MF signal, without C-propeptide
pPIC3.5K-C5A1M
P. pastoris yJC300
full length prepro-1(V) chain
pPICZ-C5A2
P. pastoris yJC300
full length prepro-2(V) chain
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