Effective and Steady Differentiation of a Clonal Derivative of P19CL6 Embryonal Carcinoma Cell Line into Beating Cardiomyocytes
Figure 3
Amplification of GATA4, Nkx2-5, and α-MHC mRNAs by RT-PCR. (a) Expression of GATA4, Nkx2-5, and α-MHC in the P19CL6 cells differentiated by the basal protocol and the P19CL6-A1 cells by the developed protocol on day 0 and 16, respectively, ((b), (c) and (d)). Densitometric quantification of the amplification levels of GATA4 (b), Nkx2-5 (c), and α-MHC (d) in the P19CL6 and P19CL6-A1 cells in the respective differentiation protocols on day 0, 3, 7, and 16. Data from three independent experiments, normalized to the levels of the GAPDH amplification products, are shown as mean SE. Comparisons between the data of P19CL6 and P19CL6-A1 cells were performed using unpaired -test. (e) Amplification of α-MHC mRNAs by RT-PCR in the P19CL6-A1 cells differentiated by the developed protocol with DMEM on day 16, and the heart ventricles of 16.5 dpc fetal and 8-week-old mice.
