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Journal of Biomedicine and Biotechnology
Volume 2010, Article ID 380561, 12 pages
http://dx.doi.org/10.1155/2010/380561
Methodology Report

Effective and Steady Differentiation of a Clonal Derivative of P19CL6 Embryonal Carcinoma Cell Line into Beating Cardiomyocytes

Department of Veterinary Anatomy, Graduate School of Life and Environmental Sciences, Osaka Prefecture University, 1-58 Rinku-Ohraikita, Izumisano, Osaka 598-8531, Japan

Received 11 August 2009; Revised 1 December 2009; Accepted 25 December 2009

Academic Editor: Leon Spicer

Copyright © 2010 Itsuki Mueller et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Linked References

  1. S. Itescu, M. D. Schuster, and A. A. Kocher, “New directions in strategies using cell therapy for heart disease,” Journal of Molecular Medicine, vol. 81, no. 5, pp. 288–296, 2003. View at Google Scholar · View at Scopus
  2. I. Kehat and L. Gepstein, “Human embryonic stem cells for myocardial regeneration,” Heart Failure Reviews, vol. 8, no. 3, pp. 229–236, 2003. View at Publisher · View at Google Scholar · View at Scopus
  3. L. Gepstein, “Derivation and potential applications of human embryonic stem cells,” Circulation Research, vol. 91, no. 10, pp. 866–876, 2002. View at Publisher · View at Google Scholar · View at Scopus
  4. S. Lev, I. Kehat, and L. Gepstein, “Differentiation pathways in human embryonic stem cell-derived cardiomyocytes,” Annals of the New York Academy of Sciences, vol. 1047, pp. 50–65, 2005. View at Publisher · View at Google Scholar · View at Scopus
  5. M. A. G. van der Heyden and L. H. K. Defize, “Twenty one years of P19 cells: what an embryonal carcinoma cell line taught us about cardiomyocyte differentiation,” Cardiovascular Research, vol. 58, no. 2, pp. 292–302, 2003. View at Publisher · View at Google Scholar · View at Scopus
  6. H. Wei, O. Juhasz, J. Li, Y. S. Tarasova, and K. R. Boheler, “Embryonic stem cells and cardiomyocyte differentiation: phenotypic and molecular analyses,” Journal of Cellular and Molecular Medicine, vol. 9, no. 4, pp. 804–817, 2005. View at Google Scholar · View at Scopus
  7. M. W. McBurney, E. M. V. Jones-Villeneuve, M. K. S. Edwards, and P. J. Anderson, “Control of muscle and neuronal differentiation in a cultured embryonal carcinoma cell line,” Nature, vol. 299, no. 5879, pp. 165–167, 1982. View at Google Scholar · View at Scopus
  8. I. S. Skerjanc, “Cardiac and skeletal muscle development in P19 embryonal carcinoma cells,” Trends in Cardiovascular Medicine, vol. 9, no. 5, pp. 139–143, 1999. View at Publisher · View at Google Scholar · View at Scopus
  9. M. W. McBurney, “P19 embryonal carcinoma cells,” International Journal of Developmental Biology, vol. 37, no. 1, pp. 135–140, 1993. View at Google Scholar · View at Scopus
  10. J. Paquin, B. A. Danalache, M. Jankowski, S. M. McCann, and J. Gutkowska, “Oxytocin induces differentiation of P19 embryonic stem cells to cardiomyocytes,” Proceedings of the National Academy of Sciences of the United States of America, vol. 99, no. 14, pp. 9550–9555, 2002. View at Publisher · View at Google Scholar · View at Scopus
  11. A. Habara-Ohkubo, “Differentiation of beating cardiac muscle cells from a derivative of P19 embryonal carcinoma cells,” Cell Structure and Function, vol. 21, no. 2, pp. 101–110, 1996. View at Google Scholar · View at Scopus
  12. Y. Ohtsu, K. Johkura, K.-I. Ito et al., “Stimulation of P19CL6 with multiple reagents induces pulsating particles in vivo,” Current Medical Research and Opinion, vol. 21, no. 5, pp. 795–803, 2005. View at Publisher · View at Google Scholar · View at Scopus
  13. F. Fathi, S. Murasawa, S. Hasegawa, T. Asahara, A. J. Kermani, and S. J. Mowla, “Cardiac differentiation of P19CL6 cells by oxytocin,” International Journal of Cardiology, vol. 134, no. 1, pp. 75–81, 2009. View at Publisher · View at Google Scholar · View at Scopus
  14. D. Kami, I. Shiojima, H. Makino et al., “Gremlin enhances the determined path to cardiomyogenesis,” PLoS ONE, vol. 3, no. 6, article e2407, 2008. View at Publisher · View at Google Scholar · View at Scopus
  15. S.-C. Choi, J. Yoon, W.-J. Shim, Y.-M. Ro, and D.-S. Lim, “5-azacytidine induces cardiac differentiation of P19 embryonic stem cells,” Experimental and Molecular Medicine, vol. 36, no. 6, pp. 515–523, 2004. View at Google Scholar · View at Scopus
  16. S. Wilton and I. Skerjanc, “Factors in serum regulate muscle development in P19 cells,” In Vitro Cellular and Developmental Biology-Animal, vol. 35, no. 4, pp. 175–177, 1999. View at Google Scholar · View at Scopus
  17. B. G. Bruneau, “Transcriptional regulation of vertebrate cardiac morphogenesis,” Circulation Research, vol. 90, no. 5, pp. 509–519, 2002. View at Publisher · View at Google Scholar · View at Scopus
  18. Y. Yamauchi, A. Harada, and K. Kawahara, “Changes in the fluctuation of interbeat intervals in spontaneously beating cultured cardiac myocytes: experimental and modeling studies,” Biological Cybernetics, vol. 86, no. 2, pp. 147–154, 2002. View at Publisher · View at Google Scholar · View at Scopus
  19. P. J. Gianakopoulos and I. S. Skerjanc, “Hedgehog signaling induces cardiomyogenesis in P19 cells,” Journal of Biological Chemistry, vol. 280, no. 22, pp. 21022–21028, 2005. View at Publisher · View at Google Scholar · View at Scopus
  20. J.-Y. Lim, W. H. Kim, J. Kim, and S. I. Park, “Involvement of TGF-β1 signaling in cardiomyocyte differentiation from P19CL6 cells,” Molecules and Cells, vol. 24, no. 3, pp. 431–436, 2007. View at Google Scholar · View at Scopus
  21. J. C. Moore, R. Spijker, A. C. Martens et al., “A P19CI6 GFP reporter line to quantify cardiomyocyte differentiation of stem cells,” International Journal of Developmental Biology, vol. 48, no. 1, pp. 47–55, 2004. View at Publisher · View at Google Scholar · View at Scopus
  22. T. Nakane, T. Satoh, Y. Inada, J. Nakayama, F. Itoh, and S. Chiba, “Molecular cloning and expression of HRLRRP, a novel heart-restricted leucine-rich repeat protein,” Biochemical and Biophysical Research Communications, vol. 314, no. 4, pp. 1086–1092, 2004. View at Publisher · View at Google Scholar · View at Scopus
  23. Y. Ohara, T. Atarashi, T. Ishibashi, A. Ohashi-Kobayashi, and M. Maeda, “GATA-4 gene organization and analysis of its promoter,” Biological and Pharmaceutical Bulletin, vol. 29, no. 3, pp. 410–419, 2006. View at Publisher · View at Google Scholar · View at Scopus
  24. C.-F. Peng, Y. Wei, J. M. Levsky, T. V. McDonald, G. Childs, and R. N. Kitsis, “Microarray analysis of global changes in gene expression during cardiac myocyte differentiation,” Physiological Genomics, vol. 9, no. 3, pp. 145–155, 2002. View at Google Scholar · View at Scopus
  25. S. Uchida, S. Fuke, and T. Tsukahara, “Upregulations of Gata4 and oxytocin receptor are important in cardiomyocyte differentiation processes of P19CL6 cells,” Journal of Cellular Biochemistry, vol. 100, no. 3, pp. 629–641, 2007. View at Publisher · View at Google Scholar · View at Scopus
  26. J. Wen, Q. Xia, C. Lu et al., “Proteomic analysis of cardiomyocytes differentiation in mouse embryonic carcinoma P19CL6 cells,” Journal of Cellular Biochemistry, vol. 102, no. 1, pp. 149–160, 2007. View at Publisher · View at Google Scholar · View at Scopus
  27. A. Alexandrovich, M. Arno, R. K. Patient, A. M. Shah, J. A. Pizzey, and A. C. Brewer, “Wnt2 is a direct downstream target of GATA6 during early cardiogenesis,” Mechanisms of Development, vol. 123, no. 4, pp. 297–311, 2006. View at Publisher · View at Google Scholar · View at Scopus
  28. E. M. McNally, R. Kraft, M. Bravo-Zehnder, D. A. Taylor, and L. A. Leinwand, “Full-length rat alpha and beta cardiac myosin heavy chain sequences. Comparisons suggest a molecular basis for functional differences,” Journal of Molecular Biology, vol. 210, no. 3, pp. 665–671, 1989. View at Publisher · View at Google Scholar · View at Scopus
  29. A. M. Wobus, T. Kleppisch, V. Maltsev, and J. Hescheler, “Cardiomyocyte-like cells differentiated in vitro from embryonic carcinoma cells P19 are characterized by functional expression of adrenoceptors and Ca2+ channels,” In Vitro Cellular and Developmental Biology-Animal, vol. 30 A, no. 7, pp. 425–434, 1994. View at Google Scholar · View at Scopus
  30. H. Brasch and H. Iven, “Inotropic and electrophysiological effects of BDF 9148, a congener of DPI 201-106, in guinea-pig atria and papillary muscles,” British Journal of Pharmacology, vol. 103, no. 4, pp. 1939–1945, 1991. View at Google Scholar · View at Scopus
  31. D. G. Wynne, P. A. Poole-Wilson, and S. E. Harding, “Incomplete reversal of β-adrenoceptor desensitization in human and guinea-pig cardiomyocytes by cyclic nucleotide phosphodiesterase inhibitors,” British Journal of Pharmacology, vol. 109, no. 4, pp. 1071–1078, 1993. View at Google Scholar · View at Scopus
  32. Y. Haraguchi, T. Shimizu, M. Yamato, A. Kikuchi, and T. Okano, “Electrical coupling of cardiomyocyte sheets occurs rapidly via functional gap junction formation,” Biomaterials, vol. 27, no. 27, pp. 4765–4774, 2006. View at Publisher · View at Google Scholar · View at Scopus
  33. C.-K. Yeung, F. Sommerhage, G. Wrobel et al., “To establish a pharmacological experimental platform for the study of cardiac hypoxia using the microelectrode array,” Journal of Pharmacological and Toxicological Methods, vol. 59, no. 3, pp. 146–152, 2009. View at Publisher · View at Google Scholar · View at Scopus
  34. R. Micheletto, M. Denyer, M. Scholl et al., “Observation of the dynamics of live cardiomyocytes through a free-running scanning near-field optical microscopy setup,” Applied Optics, vol. 38, no. 31, pp. 6648–6652, 1999. View at Google Scholar · View at Scopus
  35. D. Weisensee, T. Seeger, A. Bittner, J. Bereither-Hahn, W. Schoeppe, and I. Low-Friedrich, “Cocultures of fetal and adult cardiomyocytes yield rhythmically beating rod shaped heart cells from adult rats,” In Vitro Cellular and Developmental Biology-Animal, vol. 31, no. 3, pp. 190–195, 1995. View at Google Scholar · View at Scopus