Cell Density Plays a Critical Role in Ex Vivo Expansion of T Cells for Adoptive Immunotherapy

Figure 2

ATCs proliferate at high cell density. ATCs were activated at high density and then reseeded in fresh growth medium plus IL-2 at varying concentrations. (a) Numbers of ATCs at different cell densities. ATCs were cultured at high cell density (1×10⁵/mL) or low cell density (1×10⁴/mL) in 75 cm² flasks for 6 days. Cell number change rate (%) was monitored by direct cell counting. (b) Cell division of ATCs at different cell densities. After labeling with CFSE, ATCs were cultured at 1×10⁵/mL, 1×10⁴/mL, and 1×10³/mL in 75 cm² flasks for 6 days. Cell division was monitored by CFSE dilution in the dividing cells as analyzed by flow cytometry.

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