Cell Density Plays a Critical Role in Ex Vivo Expansion of T Cells for Adoptive Immunotherapy
Figure 2
ATCs proliferate at high cell density. ATCs were activated at high density and then reseeded in fresh growth medium plus IL-2 at varying concentrations. (a) Numbers of ATCs at different cell densities. ATCs were cultured at high cell density (/mL) or low cell density (/mL) in 75 cm2 flasks for 6 days. Cell number change rate (%) was monitored by direct cell counting. (b) Cell division of ATCs at different cell densities. After labeling with CFSE, ATCs were cultured at /mL, /mL, and /mL in 75 cm2 flasks for 6 days. Cell division was monitored by CFSE dilution in the dividing cells as analyzed by flow cytometry.