Enhancement of Human Prolactin Synthesis by Sodium Butyrate Addition to Serum-Free CHO Cell Culture

Table 2

Recombinant hPRL purification starting from 500 mL of NaBu-treated or untreated CHO-conditioned medium.

Purification step	Total protein (mg)^a		hPRL (mg)		Mass fraction
	0 mM NaBu	1 mM NaBu	0 mM NaBu	1 mM NaBu	0 mM NaBu	1 mM NaBu
Conditioned medium	64.7	66.2	0.54	1.20b	0.008	0.018
SP-Sepharose FF	2.3	3.0	0.51	1.22c	0.219	0.403
Preparative HPSEC	0.14	0.25	0.13	0.25c	0.978	1.000

aEstimated by BCA assay. bEstimated by RP-HPLC. cEstimated by HPSEC.