410405.fig.002a
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410405.fig.002b
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410405.fig.002c
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410405.fig.002d
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410405.fig.002e
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410405.fig.002f
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410405.fig.002g
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410405.fig.002h
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Figure 2: Transmission electron micrographs. (a) Satellite cell bordering a myofiber. (b) Detail showing the nucleolus mostly composed of granular component (G); whereas the dense fibrillar component (D) is less abundant, note the small fibrillar center (arrowhead). Satellite cell nuclei from adult (c, e and g) and old (d, f and h) rats. (c and d) Quadriceps muscles, anti-polymerase II (12 nm), and anti-DNA/RNA hybrid (18 nm) antibodies: both antibody probes specifically label perichromatin fibrils (arrows). Note the clustered perichromatin fibrils in (d) (arrowhead). The interchromatin granules (IG) are unlabeled. (e, f) Biceps muscles, anti-snRNP antibody: perichromatin fibrils (arrows) and interchromatin granules (IG) are labeled. (g, h) Quadriceps muscles, anti-CstF antibody: gold grains specifically label perichromatin fibrils (arrows) and RNP tails rising from perichromatin granules (inset). Note the cluster of perichromatin fibrils in (h) (arrowhead). Interchromatin granules (IGs) are unlabeled. Ch: condensed chromatin. Bars: (a) 1  𝜇 m; (b–h) 250 nm; inset 100 nm.