Dual Effect of Neutrophils on pIgR/Secretory Component in Human Bronchial Epithelial Cells: Role of TGF-
Figure 3
TGF-1 production in epithelial/neutrophil cocultures. (a) TGF-1 production was measured by ELISA in supernatants of Calu-3 cells incubated for 48 hrs with increasing numbers of neutrophils (1.25 106 to 5 106 cells). (b) Kinetics (from 2 to 72 hrs) of TGF-1 production by Calu-3 cells alone (open bars), neutrophils alone (5 106 cells, stripped bars) or epithelial cell/neutrophil cocultures (5 106 neutrophils, solid bars). The horizontal bar represents TGF-1 concentration in complete medium, containing fetal calf serum ( pg/ml, mean SD, ). Data are means SEM ( independent experiments, both for a and b). (a) as compared with control; (b) as compared with Calu-3 alone, as compared with Calu-3 at 2 hrs.
