Proteomic Studies of Cholangiocarcinoma and Hepatocellular Carcinoma Cell Secretomes
SDS-PAGE analysis of secretomes obtained from five cell lines. HuCCA-1, HCC-S102, HepG2, SK-Hep-1, and Alexander are shown in order from left to right, with MW standards in the leftmost lane. Each gel was cut into 0.5 mm slices, numbered as shown from the top of the gel to the dye front. Proteins in each slice were reduced, alkylated, digested with trypsin, and subjected to LC/MS/MS analysis.