Research Article

Proteomic Studies of Cholangiocarcinoma and Hepatocellular Carcinoma Cell Secretomes

Figure 4

Two-dimensional gel electrophoresis of conditioned media from HuCCA-1 stained with Coomassie Blue R-250 (a) and immunoblot labeled with the antibody to lipocalin 2 (b). The pI and MW of spot numbers 1, 2, 3, and 4 were approximately 6.0/23.5, 6.5/23.5, 7.3/23, and 8.7/22.5 respectively.