Differential Proteome Analysis of the Preeclamptic Placenta Using Optimized Protein Extraction
Two-dimensional separation of precipitated proteins (100 g) in PE placenta solubilized in (a) urea/CHAPS and (b) Hepes buffer. Precipitation procedures (1) dichloromethane/methanol, (2) TCA, (3) TCA followed by ethanol wash, (4) acetone, (5) acidified acetone, and (6) ethanol. The gels were stained with silver. Arrows on lower right image represent directions for increasing Mw and pI. Directions are the same for all gels.
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