BioMed Research International / 2010 / Article / Fig 3

Methodology Report

Differential Proteome Analysis of the Preeclamptic Placenta Using Optimized Protein Extraction

Figure 3

Two-dimensional separation of precipitated proteins (100  𝜇 g) in PE placenta solubilized in urea/CHAPS. Segments show differential expression pattern in consequence of precipitation procedures. (a) dichloromethane/methanol, (b) TCA, (c) TCA followed by ethanol wash, (d) acetone, (e) acidified acetone, and (f) ethanol. Circles indicate landmarks for orientation. The gels were stained with silver.

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