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Journal of Biomedicine and Biotechnology
Volume 2010 (2010), Article ID 475432, 8 pages
http://dx.doi.org/10.1155/2010/475432
Research Article

Polyploidization Facilitates Biotechnological In Vitro Techniques in the Genus Cucumis

Department of Botany, Faculty of Science, Palacký University in Olomouc, Šlechtitelů 11, 783 71 Olomouc, Czech Republic

Received 28 June 2010; Revised 27 September 2010; Accepted 19 November 2010

Academic Editor: Neal Stewart

Copyright © 2010 Dagmar Skálová et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

Prezygotic interspecific crossability barrier in the genus Cucumis is related to the ploidy level of the species (cucumber (C. sativus), x = 7 ; muskmelon (C. melo) and wild Cucumis species, x = 1 2 ). Polyploidization of maternal plants helps hybridization among other Cucumis species by overcoming prezygotic genetic barriers. The main objective of this paper is to compare the results of several methods supporting interspecific crosses in cucumber without and with polyploidization (comparison between diploid (2x) and mixoploid (2x/4x) cucumber maternal plants). Mixoploid plants were obtained after in vivo and in vitro polyploidization by colchicine and oryzalin. Ploidy level was estimated by flow cytometry. Embryo rescue, in vitro pollination, and isolation of mesophyll protoplast were tested and compared. Positive effect of polyploidization was observed during all experiments presented by higher regeneration capacity of cultivated mixoploid cucumber embryos, ovules, and protoplasts. Nevertheless, the hybrid character of putative hybrid accessions obtained after cross in vivo and in vitro pollination was not confirmed.