Polyploidization Facilitates Biotechnological In Vitro Techniques in the Genus Cucumis

Table 2

Cultivation media used in this study.

Medium	Composition of media	References
OK*	MS medium + 20 mg/l ascorbic acid, 0.01 mg/l IBA, 0.01 mg/l BA, 20 g/l sucrose, 8 g/l agar	[30]
ON*	MS medium + 1 g/l casein hydrolysate, 0.01 mg/l IBA, 0.01 mg/l BA, 20 g/l sucrose, 6 g/l agar	[30]
CW*	MS medium + 5% coconut water, 200 mg/l α-glutamine, 0.01 mg/l IBA, 0.01 mg/l BA, 60 g/l sucrose, 6 g/l agar	[30]
GA*	MS medium + 0.3 mg/l GA3, 0.01 mg/l IBA, 0.01 mg/l BA, 20 g/l sucrose, 8 g/l agar	[30]
YS**	600 mg/l Ca(NO3)2 x H2O, 100 mg/l H3BO3, 80 g/l sucrose, 8 g/l agar	[21, 31]
CP**	MS medium + 9.5 mg/l glycine, 500 mg/l casein hydrolysate, 40 g/l sucrose, 4 mg/l IAA, 0.5 mg/l KIN, 5 mg/l GA3, 40 g/l sucrose, 8 g/l agar	[21, 31]
N**	N medium + 500 mg/l casein hydrolysate, 50 g/l sucrose, 8 g/l agar	[21, 31]
MSN***	MS medium + 30 g/l sucrose, 0.5 mg/l NAA, 1 mg/l KIN, 8 g/l agar	[31]

Explanatory Notes. OK, ON, CW, GA, YS, CP, N, and MSN: the abbreviations for media used during the experiments; MS medium [32]; N medium [33]; ∗media used for embryo rescue after in vivo pollination; ∗∗media used for in vitro pollination; ∗∗∗medium used for embryogenesis after in vitro pollination; IBA: indole-3-butyric acid; BA: benzyladenine; GA₃: gibberellic acid; KIN: kinetin; NAA: α-naphthalene-acetic acid.