Comparison of the Immunoregulatory Function of Different Constituents in Radix Astragali and Radix Hedysari

Table 2

Accurate mass measurements of the compounds in MGHQ, MJHQ, and HQ as revealed by UPLC/TOF-MS analysis in negative mode.

Peak no.	𝑡𝑅 (min)	Selected ion	m/z	Compound identification
1	4.33	[M+HCOOH−H]^−	491.1221	calycosin-7-O-𝛽-D-glucoside
2	6.66	[M+HCOOH−H]^−	475.1225	ononin
3	6.75	[M+HCOOH−H]^−	625.2927	naringin
4	7.19	[M+HCOOH−H]^−	507.1475	(6aR, 11aR)-9,10-dimethoxypterocarpan-3-O-𝛽-D-glucopyranoside
5	7.86	[M−H]^−	463.1603	(3R)-2^-hydroxy-3^,4^-dimethoxyisoflavan-7-O-𝛽-D-glucopyranoside
6	8.93	[M−H]^−	283.061	calycosin
7	10.46	[M+HCOOH−H]^−	957.5071	soyasaponin II
8	11.69	[M+HCOOH−H]^−	829.462	astragaloside IV
9	12.16	[M−H]^−	267.0686	formononetin
10	12.47	[M+HCOOH−H]^−	871.4798	astragaloside II
11	12.70	[M+HCOOH−H]^−	667.4065	astramembrannin II
12	13.09	[M+HCOOH−H]^−	871.4722	isoastragaloside II
13	14.28	[M+HCOOH−H]^−	913.4913	astragaloside I
14	14.37	[M−H]^−	941.5078	soyasaponin I
15	14.60	[M+HCOOH−H]^−	913.4836	isoastragaloside I
16	16.05	[M+HCOOH−H]^−	955.4894	acetylastragaloside I
17	16.28	[M+HCOOH−H]^−	315.2524	medicarpin