Testosterone Depletion by Castration May Protect Mice from Heat-Induced Multiple Organ Damage and Lethality

Table 2

Mean (±SEM) number of TUNEL-positive cells per tissue section for different groups of mice.

Treatment groups	Hypothalamus spleen kidney
(1) Normothermic controls (NCs)	0
(2) Sham-operated, vehicle-treated heatstroke (SOH) mice	344±107^∗
(3) Castrated, vehicle-treated heatstroke (CVH) mice	70±17^∗∗
(4) Castrated, testosterone-treated heatstroke (CTH) mice	312±95^∗∗∗

* 𝑃<.05 in comparison to group 1; **𝑃<.05 in comparison to group 2;
*** 𝑃<.05 in comparison to group 3. Vehicle or testosterone was administered 14 days before whole body heating, and the tissue section was obtained for TUNEL assay 2.5 h post-WBH. Data are means ± SEM of 7 mice per group