Cytokines and Growth Factors Stimulate Hyaluronan Production: Role of Hyaluronan in Epithelial to Mesenchymal-Like Transition in Non-Small Cell Lung Cancer
Figure 4
Increased HA production promotes cell invasion and induces enhanced MMP2 and MMP9 activities in H358 cells. (a) Invasive capacity of HAS3 and vector—only control transfectants were determined using invasion chamber assay (BD Bioscience) as outlined in Material and Method. (b) mRNA expression for MMP2 and MMP9 were determined by quantitative real time RT-PCR. (c) Following 48 hours incubation in serum-free media, media was collected and gelatinase activities for MMP2 and MMP9 were assayed by gelatin zymography. The experiments were repeated twice. The data shown for (a) and (b) is a representative. (Values are , , .)
