The Protection of Hepatocyte Cells from the Effects of Oxidative Stress by Treatment with Vitamin E in Conjunction with DTT
Changes in the fluorescence intensity of intracellular calcium in TBH-treated hepatocytes. Using confocal microscopy, the changes of cell morphology were also photographed before (a), 12 (c), 18 (e), 30 (g), and 60 min (i) after 2.0 mM TBH treatment. At the same time, the changes of fluorescence intensity of intracellular calcium were photographed before (b), 12 (d), 18 (f), 30 (h) and 60 min (j) after 2.0 mM TBH treatment. Pseudodensity scale indicates fluorescence intensity in arbitrary units. Arrows indicate the cells with bleb. Bar, 20 m.