The Protection of Hepatocyte Cells from the Effects of Oxidative Stress by Treatment with Vitamin E in Conjunction with DTT
Figure 3
The effect of vitamin E and DTT on the concentration of intracellular calcium in TBH-treated hepatocytes. (a) Changes in concentration of intracellular calcium were determined in the cells treated with 2.0 mM TBH (control), with 2.0 mM TBH by the pretreatment with 100 M vitamin E for 20 h, or with 15 mM EGTA for 15 min. (b) Changes in concentration of intracellular calcium were determined in the cells treated with 2.0 mM TBH (control) or with 2.0 mM TBH by the pretreatment with 5.0 mM DTT for 15 min. The results were based on three separate experiments, and the values are expressed as mean SD. Treatment means in the same time not sharing the same superscripts differ significantly (5).
