The Protection of Hepatocyte Cells from the Effects of Oxidative Stress by Treatment with Vitamin E in Conjunction with DTT

Table 2

Effect of vitamin E and DTT on the loss of membrane protein thiols in TBH-treated hepatocytes 60 min after treatment.

Treatment	Protein thiol level (%)
	Membrane	Cytosol
Untreated	100^a	100^a
TBH (1.0 mM)	78.7±4.7^b	83.6±6.9^b
TBH (2.0 mM)	59.0±8.3^c	71.1±7.9^c
Vitamin E (100 𝜇M) + TBH (2.0 mM)	85.4±13.2^a	76.8±2.9^c
EGTA (15 mM) + TBH (2.0 mM)	76.1±3.2^b	83.6±2.1^b
DTT (5 mM) + TBH (2.0 mM)	75.7±3.1^b	96.4±5.5^a
Vitamin E (100 𝜇M) + DTT (5 mM) + TBH (2.0 mM)	114.2±8.8^a	92.2±10.0^a

Values are expressed as mean ± SD (𝑛=3-4). Means in the same column not sharing the same superscripts differ significantly (𝑃<.05).