Regression of Human Prostate Tumors and Metastases in Nude Mice following Treatment with the Recombinant Oncolytic Vaccinia Virus GLV-1h68
Figure 5
Effects of the GLV-1h68 virus on metastases formation in the regional lymph nodes. (a) Percentage of PC-3 positive regional lymph nodes in tumor-bearing mice at day 21 after PBS or virus injection. Fisher’s exact test (GraphPad Prism software, San Diego, USA) was used for statistical analysis. The value of was considered significant. GLV-1h68 : 1h68. (b) RT-PCR analysis of tumors and regional lymph nodes of PC-3 xenograft mice injected either with PBS or with 5 106 pfu of GLV-1h68 (1h68) using primers for human beta-actin (lines 1; indicating PC-3 cancer cells, PCR-fragment 205 bp), mouse beta-actin (lines 2; positive control, PCR-fragment 216 bp), and vaccinia virus (lines 3; PCR-fragment 204 bp). ln: lymph node of two different mice.
