Microarray Detection Call Methodology as a Means to Identify and Compare Transcripts Expressed within Syncytial Cells from Soybean (Glycine max) Roots Undergoing Resistant and Susceptible Reactions to the Soybean Cyst Nematode (Heterodera glycines)
Figure 8
Detection calls. (a, c, e, g, i) Gene counts per sample. (b, d, f, h, j) Functional categories for the histograms. P: pericycle; I3: incompatible syncytium-3 dpi; C3: compatible syncytium 3 dpi; C8: compatible syncytium-8 dpi; DE: differentially expressed. The functional categories are as follows: A: Cell Growth & Division; B: Cell Structure; C: Disease & Defense; D: Energy; E: Intracellular Traffic; F: Metabolism; G: No Homology to Known Proteins; H: Post-Transcription; I: Protein Destination & Storage; J: Protein Synthesis; K: Secondary Metabolism; L: Signal Transduction; M: Transcription; N: Transporter; O: Transposon; P: Unclassified-Hypothetical Protein NOT Supported by cDNA; Q: Unclassified-Hypothetical Protein Supported by cDNA; R: Unclassified-Protein with Unknown Function. (a) Venn diagram depicting P versus I3. The three portions of the Venn diagram show transcripts for P-present-unique; common-present; I3-present-unique. (b) Functional categorization depicting P versus I3. The seven histograms (per functional category) are P-present-unique; common-present; I3-present-unique. The remaining four transcript identifications represent comparisons between those that measured DE [26] and those that were present: I3-present & DE; I3-DE-induced; I3 dpi-DE-suppressed; I3-present & not DE. (c) Venn diagram depicting P versus C3. The three portions of the Venn diagram show transcripts for P-present-unique; common-present; C3-present-unique. (d) Functional categorization depicting P versus C3. The seven histograms (per functional category) are P-present-unique; common-present; C3-present-unique. The remaining four transcript identifications represent comparisons between those that measured DE [26] and those that were present: C3-present & DE; C3-DE-induced; C3-DE-suppressed; C3-present & not DE. (e) Venn diagram depicting P versus C8. The three portions of the Venn diagram show transcripts for P-present-unique; common-present; C8 present-unique. (f) Functional categorization depicting P versus C8. The seven histograms (per functional category) are P-present-unique; common-present; C8-present-unique. The remaining four transcript identifications represent comparisons between those that measured DE [26] and those that were present: C8-present & DE; C8-DE-induced; C8-DE-suppressed; C8-present & not DE. (g) Venn diagram depicting C3 versus I3. The three portions of the Venn diagram show transcripts for C3-present-unique; common-present; I3-present-unique. (h) Functional categorization depicting C3 versus I3. The eight histograms (per functional category) are C3-present-unique; common-present; I3-present-unique. The remaining five transcript identifications represent comparisons between those that measured DE [26] and those that were present: I3-present & DE; I3-DE-induced; I3-DE-suppressed; I3-present & not DE; C3-present & not DE. (i) Venn diagram depicting C3 versus C8. The three portions of the Venn diagram show transcripts for C3-present-unique; common-present; C8-present-unique. (j) Functional categorization depicting C3 versus C8. The seven histograms (per functional category) are C3-present-unique; common-present; C8-present-unique. The remaining four transcript identifications represent comparisons between those that measured DE [26] and those that were present: C8-present & DE; C8-DE-induced; I3-DE-suppressed; C8-present & not DE.
