Construction and Characterization of Insect Cell-Derived Influenza VLP: Cell Binding, Fusion, and EGFP Incorporation

Figure 1

Construction of pFastbac Dual plasmid. Viral genes were flanked two different restriction sites while PCR amplification and ligated with pFastbac Dual vector as indicated. HA, M1 and M2, NA genes were paired and cloned in separate pFastbac Dual vectors and used for bacmids recombination. When constructing pFastbac Dual vectors for HA^- and NA-EGFP VLPs, HA gene was omitted, and NA was replaced with NA-EGFP fusion gene, respectively. Tn7L and Tn7R were left and right arms of bacterial transposon Tn7 for gene recombination.