Construction and Characterization of Insect Cell-Derived Influenza VLP: Cell Binding, Fusion, and EGFP Incorporation
Figure 5
Membrane fusion of VLPs with Jurkat cells. Jurkat cells were mixed with R18-labeled VLPs for 10 min and then collected for detection of the membrane fusion. The fusion process was initiated by lowering the external pH to 5. (a) shows HA+ VLP binding, HA- VLP binding, HA+ VLP bound to cells at pH5 for 2 min, HA- VLP bound to cells at pH5 for 2 min, from left to right. Graph in (b) depicts the time profile of VLP fusion with plasma membrane triggered by acidification at 1 min as indicated. The autofluorescence of Jurkat cells obtained as a control was subtracted from the intensity value.
