BioMed Research International / 2010 / Article / Fig 3

Research Article

The Immunoexpression of FSH-R in the Ductuli Efferentes and the Epididymis of Men and Rat: Effect of FSH on the Morphology and Steroidogenic Activity of Rat Epididymal Epithelial Cells In Vitro

Figure 3

The 5th-day culture of epididymal epithelial cells: epididymal epithelial cells cultured in medium with DHT. The cells form confluent monolayer. The cytoplasm is rich in PAS-positive substances (red arrows) and glycogen (a) and lipid droplets (black arrows) (b). Epididymal epithelial cells are cultured without DHT. The cells stellate in the shape connected by cytoplasmic processes. The cytoplasm are poor in PAS-positive substances and glycogen (c) and lipid droplets (black arrows) (d). Epididymal epithelial cells are cultured without DHT in supplementation with 0.8 IU/mL FSH. The cytoplasm is poor in PAS-positive substances (red arrow) and in glycogen (e), and rich in lipid droplets (black arrow) (f). (a), (c), and (e)—PAS; (b), (d), and (f)—ORO: Scale bar: 20  𝜇 m.
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