Decrease of Klotho in the Kidney of Streptozotocin-Induced Diabetic Rats

Figure 5

Changes in Klotho expression levels in cultured MDCK cells. (a) Western blot analysis of Klotho expression in normal glucose (5 mmol/L) medium in MDCK cells treated with H₂O₂. Upper picture shows the protein level of Klotho or actin in MDCK cells. ^∗∗𝑃<.001 compared to MDCK cells without H₂O₂ treatment. Western blot analysis was used to detect Klotho (b) expression in MDCK cells cultured with normal glucose (5 mmol/L; control) or high glucose (40 mmol/L glucose; HG) medium with or without tiron (10 mmol/L) treatment. Upper picture shows the protein level of Klotho or actin in MDCK cells. Quantification of protein levels indicated as means with SE (𝑛=4 per group) in each column are presented in the lower panel. ^∗∗𝑃<.001 compared to control.

(a)

(b)