WT1-specific TCR gene was modified to express more efficiently (hybrid human-murine TCR, Cys-mutant TCR). Patients’ PBL transduced WT1-specific TCR could reject the engraftment of autologous CML-BC cells in NOD/SCID mice model.
conventional vector: 0.6% in total CD8+ T cells by tetramer (nonstimulated) optimised vector: 6% in total CD8+ T cells by tetramer (nonstimulated)
HA-2-specific TCR gene was transduced into CMV-specific CTL clone to exploit the viral specific response in vivo for the long-term persistence of target TCR transfectants.