BioMed Research International / 2010 / Article / Fig 4

Research Article

The Vacuolar-Type -ATPase in Ovine Rumen Epithelium is Regulated by Metabolic Signals

Figure 4

Immunolocalization of N a + / K + -ATPase 𝛼 -subunit (A, green) and of v H + -ATPase B subunit (B, green) in ovine REC. Monoclonal mouse antibodies against sheep N a + / K + -ATPase 𝛼 -subunit or yeast v H + -ATPase 60 kDa subunit, and an Alexa fluor 488 conjugated secondary antibody were used. The nuclei are counterstained with DAPI (blue). Compared to control conditions (Ba) glucose substitution with 2-DOG (Bb) and complete MI (glucose substitution with 2-DOG and antimycin A application; Bc) induced loss of membrane staining and appearance of diffuse, cytosolic staining of v H + -ATPase B subunits. In contrast, the N a + / K + -ATPase was always localized to the cell membrane and not influenced by metabolic inhibition (A a–c).

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