The Vacuolar-Type -ATPase in Ovine Rumen Epithelium is Regulated by Metabolic Signals
Immunolocalization of -ATPase -subunit (A, green) and of -ATPase B subunit (B, green) in ovine REC. Monoclonal mouse antibodies against sheep -ATPase -subunit or yeast -ATPase 60 kDa subunit, and an Alexa fluor 488 conjugated secondary antibody were used. The nuclei are counterstained with DAPI (blue). Compared to control conditions (Ba) glucose substitution with 2-DOG (Bb) and complete MI (glucose substitution with 2-DOG and antimycin A application; Bc) induced loss of membrane staining and appearance of diffuse, cytosolic staining of -ATPase B subunits. In contrast, the -ATPase was always localized to the cell membrane and not influenced by metabolic inhibition (A a–c).
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