Regulation of P-Glycoprotein in Renal Proximal Tubule Epithelial Cells by LPS and TNF-α
Figure 5
Role of NF-κB in modulation of P-gp activity in GERP cells. ((a) ) P-gp transport activity in GERP cells after exposure to 10 ng/mL TNF-α in combination with 5 M of the selective IKK inhibitor IMD-0354 compared to control (cells exposed to culture medium). ((b) ) P-gp transport activity in GERP cells after exposure to 10 ng/mL TNF-α in combination with 1.8 M of a cell-permeable inhibitor peptide that interferes with NF-κB nuclear translocation, SN50, for 24 hours compared to control. Data are expressed as mean SEM. Significantly different compared to control cells (; , : ) or to TNF-α (:). Treatment with SN50 alone showed no, but with IMD-0354 alone did show significant difference compared to control.
