BioMed Research International / 2010 / Article / Fig 2

Research Article

Ulvan, a Sulfated Polysaccharide from Green Algae, Activates Plant Immunity through the Jasmonic Acid Signaling Pathway

Figure 2

Comparison of M. truncatula transcriptomic responses to ulvan, BTH, and MeJA. M. truncatula plants were treated by ulvan (ethanol-insoluble fraction, 1 mg ml-1), MeJA (500  𝜇 M), BTH (40  𝜇 g ml-1), or Actilandes (0.01%) as the control. (a) Venn diagrams showing the total number of genes up-regulated (left) or down-regulated (right) upon ulvan, BTH, and MeJA treatments and their respective overlaps. Data obtained at 1–2 and 6 days after treatment are mixed. Only genes with a 𝑃 -value . 0 5 and a | l o g ( 2 ) r a t i o | 0 . 5 8 4 were selected. (b) Trypsin protease inhibiting activity measured on M. truncatula leaves harvested 6 days after treatment. Activity is expressed as 𝜇 g of trypsin inhibited per  mg of protein extracted from the leaves. The average of measures done on three biological replicates is represented. (c) Measurement of the SA, JA, and ABA contents of M. truncatula leaves harvested 1, 2, and 6 days after treatment using UPLC-ESI-MS/MS. Mean of the relative amount after ulvan treatment compared to Actilandes, used as control, was calculated from three experiments and represented on the graph. Mean for each treatment was compared to the control mean using a Student’s t-test. Treatment associated to a 𝑃 - v a l u e . 0 5 or . 0 1 was marked, respectively, by * or **.

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