Figure 2: T cell responses measured on splenocytes (a) and BM-derived cells (b) at 90 days from immunizations. Results from one representative experiment out of three independent experiments are shown. IFN- 𝛾 ELISPOT was performed on cells stimulated overnight with either H- 2 d restricted specific Envelope JR-9mer peptide or H- 2 d restricted unrelated GFP-9mer peptide. IFN- 𝛾 producing T cells are expressed as SFC/106 cells after background subtraction (see Section 2). The error bars indicate the standard deviation among four mice of the same group, and 𝑃 -values are indicated for intergroup comparisons.